General Description

LevelS-1 / C-1
Discovered? / A. robustus
Molecular formulaC₁₀H₂₆N₄
Precursor 1 [M+H]⁺203.22302
Precursor 2 [M+2H]²⁺102.11515
Precursor 3
Precursor HDX 1 [M(D₆)+D]⁺210.26696
Precursor HDX 2 [M(D₆)+2D]²⁺106.14026
Precursor HDX 3
Rt HDX0.98

Calculated MS/MS fragments


Additional MS/MS fragments


Recorded MS/MS spectra

Data203.22302Spermine 4HCl (CAS 306-67-2)FlukaY. M. Forster
Data203.22302PA334A. apertaFauna Laboratories Ltd., KAZY. M. Forster
DataHDXPA334A. apertaFauna Laboratories Ltd., KAZY. M. Forster
Data203.22302Ariadna sp.Spider Pharm, USAY. M. Forster
DataHDXAriadna sp.Spider Pharm, USAY. M. Forster
Data203.22302PA334Hololena sp.Spider Pharm, USAY. M. Forster
DataHDXPA334Hololena sp.Spider Pharm, USAY. M. Forster
Data203.22302PA334L. cornutusSpider Pharm, USAY. M. Forster
DataHDXPA334L. cornutusSpider Pharm, USAY. M. Forster
Data203.22302P. bistriataProf. Dr. Wagner Ferreira dos Santos, BRAY. M. Forster
DataHDXP. bistriataProf. Dr. Wagner Ferreira dos Santos, BRAY. M. Forster
Data203.22302PA334P. mexicanusSpider Pharm, USAY. M. Forster
Data203.22302P. tristisSpider Pharm, USAY. M. Forster
DataHDXP. tristisSpider Pharm, USAY. M. Forster


Spider venoms: Biochemical aspectsJ. D. McCrone, Am. Zoologist 1969, 9, 153-156A. robustusReviewLink
Analysis of the venom of the Sydney funnel-web spider, Atrax robustus using gas chromatography mass spectrometryP. H. Duffield, A. M. Duffield, P. R. Carroll, D. Morgans, Biomed. Mass. Spectrom. 1979, 6, 3, 105-108A. robustusGC-MSLink
Polyamines in some tarantula venomsS. G. Cabbiness, C. W. Gehrke, K. C. Kuo, T. K. Chan, J. E. Hall, S. A. Hudiburg, G. V. Odell, Toxicon 1980, 18, 681-683div.Link
Purification and characterization of two classes of neurotoxins from the funnel web spider, Agelenopsis apertaW. S. Skinner, M. E. Adams, G. B. Quistad, H. Kataoka, B. J. Cesarin, F. E. Enderlin, D. A. Schooley, J. Biol. Chem. 1989, 264, 2150-2155Activity-studiesLink
Determination of biogenic amines in spider venom glands of nine typical Japanese species and chromatographic elution pattern analysis of venomous componentsK. Hagiwara, A. Tokita, A. Miwa, N. Kawai, Y. Murata, A. Uchida, T. Nakajima, Jpn. J. Sanit. Zool. 1991, 42, 2, 77-84div.HPLCLink
Novel linear and branched polyamines in the extremely thermophilic eubacteria Thermoleophilum, Bacillus and HydrogenobacterK. Hamana, M. Niitsu, S. Matsuzaki, K. Samejima, Y. Igarashi, T. Kodama, Biochem. J. 1992, 284, 741-747HPLC, GCLink
The components of the venom of a spider Scodra griseipes. 1. Analysis of low molecular weight products using gas chromatography/ mass spectrometryC. Lange, C. Paris, M.-L. Celerier, Rapid Commun. Mass Spectrom. 1992, 6, 289-292S. calceatum griseipesGC-MSLink
Pharmacological identification of a novel Ca2+ channel in chicken brain synaptosomesP. M. Lundy, M. G. Hamilton, R. Frew, Brain Research 1994, 643, 201-210Review, Activity-studiesLink
Block of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors by polyamines and polyamine toxinsM. S. Washburn, R. Dingledine, J. Pharmacol. Exp. Ther. 1996, 278, 669-678Activity-studiesLink
Interactions of polyamines with ion channelsK. Williams, Biochem. J. 1997, 325, 289-297Activity-studiesLink
Polyamine analysis for chemotaxonomy of thermophilic eubacteria: Polyamine distribution profiles within the orders Aquificales, Thermotogales, Thermodesulfobacteriales, Thermales, Thermoanaerobacteriales, Clostridiales and BacillalesR. Hosoya, K. Hamana, M. Niitsu, T. Itho, J. Gen. Appl. Microbiol. 2004, 50, 271-287HPLC, GCLink
New linear polyamine derivatives in spider venomsM. Tzouros, S. Chesnov, S. Bienz, M. Hesse, L. Bigler, Toxicon 2005, 46, 350-354A. aperta & H. curtaAPCI-MS/MS (ns)Link
Molecular components and toxicity of the venom of the solitary wasp, Anoplius samariensisM. Hisada, H. Satake, K. Masuda, M. Aoyama, K. Murata, T. Shinada, T. Iwashita, Y. Ohfune, T. Nakajima, Biochemical and Biophysical Research Communications 2005, 330, 1048-1054LC-ESI-MSLink
Structure elucidation of polyamine toxins in the venom of the spider Larinioides foliumS. Eichenberger, L. Bigler, S. Bienz, Helv. Chim. Acta 2007, 61, 161-164L. patagiatusPA343 as polyamine backbone in APALink
Mass spectrometric characterisation and quantification of selected low molecular mass compounds from the venom of Haplopelma lividum (Theraphosidae)S. Moore, W. F. Smyth, V. A. Gault, E. O’Kane, S. McClean, Rapid Commun. Mass. Spectrom. 2009, 23, 1747-1755C. lividumHPLC-ESI-MS/MSLink
Venom composition and strategies in spiders: Is everything possible?L. Kuhn-Nentwig, R. Stöcklin, W. Nentwig, Adv. Insect Physiol. 2011, 40, 1-86.div.ReviewLink
A template approach for the characterization of linear polyamines and derivatives in spider venomM. Tzouros, S. Chesnov, L. Bigler, S. Bienz, Eur. J. Mass Spectrom. 2013, 19, 57-69A. aperta & H. curtaAPCI-MS/MS (ns)Link
Elucidation of the structure and synthesis of neuroprotective low molecular mass components of the Parawixia bistriata spider venomY. M. Forster, J. L. Green, A. Khatiwada, J. L. Liberato, P. A. Narayana Reddy, J. M. Salvino, S. Bienz, L. Bigler, W. Ferreira dos Santos, A. C. K. Fontana, ACS Chem Neurosci., 2020P. bistriataESI-MS/MSLink
Low molecular mass compounds in spider venomY. M. Forster, S. Bienz, L. Bigler, 2020, in preparationdiv.Link

Spider species

Spider speciesFamilyDiscovered
Agelena labyrinthicaAgelenidae1991 / K. Hagiwara
Agelena limbataAgelenidae1991 / K. Hagiwara
Agelenopsis apertaAgelenidae2005 / M. Tzouros
Allagelena opulentaAgelenidae1991 / K. Hagiwara
Aphonopelma emilia*Theraphosidae1980 / S. G. Cabbiness
Aphonopelma hentziTheraphosidae1980 / S. G. Cabbiness
Aphonopelma sp.Theraphosidae1980 / S. G. Cabbiness
Ariadna sp.Segestriidae2020 / Y. M. Forster
Atrax robustusAtracidae? / ?
Cheiracanthium japonicumCheiracanthiidae1991 / K. Hagiwara
Cyriopagopus lividumTheraphosidae2005 / S. Moore
Dolomedes sulfureusPisauridae1991 / K. Hagiwara
Hololena curtaAgelenidae2005 / M. Tzouros
Hololena sp.Agelenidae2020 / Y. M. Forster
Larinioides cornutusAraneidae2020 / Y. M. Forster
Loxosceles rufescensSicariidae1991 / K. Hagiwara
Neoscona nauticaAraneidae1991 / K. Hagiwara
Nephila clavataAraneidae1991 / K. Hagiwara
Parawixia bistriataAraneidae2020 / Y. M. Forster
Physocyclus mexicanusPholcidae2020 / Y. M. Forster
Plectreurys tristisPlectreuridae2020 / Y. M. Forster
Stromatopelma calceatum griseipesTheraphosidae1992 / C. Lange